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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become noticed in several tumors, which ends up in chromosome instability, and so could contribute to tumorigenesis. This protein was found to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a essential job in promoting cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are actually described. Two more splice variants have been reported but thorough nucleotide sequence data will not be however out there.
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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide usually has the epitope recognized with the antibody.
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This information outlines additional doable optimization of cellular and immunoassay parameters to be certain the best possible effects are...
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This information outlines additional attainable optimization of mobile and immunoassay parameters to make certain the very best outcomes are...
Antibodies sure to the blocking peptide no more bind for the epitope within the goal protein. By comparing the staining from the blocked antibody as opposed to the antibody on your own, you can see which staining is precise.
Focus on Information Cyclin E belongs to your extremely conserved cyclin family members, whose customers are characterized by a remarkable periodicity in protein abundance in the cell cycle. Cyclins function as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation designs which lead into the temporal coordination of each mitotic party. Cyclin E types a fancy with and capabilities as a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S section boundary and it is degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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